Regulatory

Part:BBa_K112402:Design

Designed by: Christina Brown   Group: iGEM08_UC_Berkeley   (2008-10-21)

promoter for FabA gene - Membrane Damage and Ultrasound Senstitive


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

pBca1256-K112402

Biobricking of promoter for fabA protein 
 PCR fabA-F and fabA-R on MG1655 	  (285 bp, EcoR1/BamH1)
 Sub into pBca1256	                  (EcoR1/BamH1 910+2472bp, L)
 Product is pBca1256-K112402
 ----------------------------
cb0005-fabA-F Forward Biobricking   cgataGAATTCatgAGATCTggccattacgttggctgaac 
cb0006-fabA-R Reverse Biobricking   cgtagGGATCCgaagttaccacccgtttcgg


Source

cloned from E. coli MG1655 genome

References